Getting Waypoint DNA Right for SCNT
Whole blood must be carefully isolated into mononuclear cells before cryopreservation
The Ficoll-Paque procedure is a widely used method for isolating mononuclear cells (lymphocytes and monocytes) from whole blood or other biological samples. This technique leverages the principle of density gradient centrifugation to separate cells based on their size and density. Here's a step-by-step outline of the process: